Next-generation sequencing

In recent years , genomics has developed rapidly with the application of next-generation sequencing technology .

最近几年中，随着下一代测序技术的应用，基因组学已经得到了快速发展。

This review discusses applications of next-generation sequencing technologies in functional genomics research and highlights the transforming potential these technologies offer .

这篇综述讨论了下一代测序技术在功能基因组学研究中的应用，并强调了这些技术所提供的转化潜能。

The throughput of the next-generation sequencing technology is very high .

第二代测序技术的通量很高。

The new reference material is for tests that use advanced technology - " next-generation sequencing " - to analyze a person 's DNA .

新推出的基准材料适用于采用了先进的“下一代测序”技术来分析个人DNA的检测。

These data are generated by the research community using high-throughput technologies like microarrays and , more recently , next-generation sequencing .

这些数据是由研究团体使用高通量技术产生的，像微阵列技术，和最近的，下一代测序。

However , the next-generation sequencing generates vast amounts of data , mining useful information from these data with bioinformatics tools becomes current urgent problem .

然而如何利用生物信息学工具从新一代测序产生的海量数据中挖掘有效的信息，成为目前急需解决的问题。

They used next-generation sequencing to establish the order of letters on the two Y chromosomes and then compared these to the Y chromosome reference sequence .

他们利用next-generation测序技术来确定这两个Y染色体的核苷酸序列，然后同对照序列进行比对。

Hopper , A.We have recently seen rapid growth of research capacity in next-generation sequencing , high-throughput genotyping and other intensive data-collection methods .

我们最近看到了在下一代测序，高通量基因分型和其他密集的数据收集方法的研究能力的快速增长。

As entering into the 21st century , the emergence of the next-generation sequencing platforms led to resurgence the research in the whole-genome assembly algorithms and software .

进入二十一世纪以来，新一代测序技术的出现使得到全基因组的拼接组装算法备受关注。

The tools are extremely efficient and allow the user to compare large datasets ( e.g. , next-generation sequencing data ) with both public and custom genome annotation tracks .

该工具特别高效，并允许用户用公开的和定制的基因组注释轨道比较大的数据集（例如，下一代测序数据）。

The next-generation sequencing technology has advantages of high-throughput , low-cost and so on . It has been widely used in the fields of animal and plant genome sequencing and gene expression .

新一代测序技术具有高通量、低成本等优势，在动物和植物的基因组测序及基因表达研究中被广泛应用。

With the development of the next-generation sequencing technology , whole genome and exome sequencing developed rapidly and provide an opportunity for us to deal with the problem caused by GWAS .

而新一代测序技术的进步，促进了全基因组测序和全基因组外显子测序的快速发展，为解决上述问题提供了契机。

From the invention of Sanger sequencing technology in 1977 , to the invention of the next-generation sequencing technology in 2005 , DNA sequencing mainly uses Sanger sequencing technology .

从1977年Sanger测序技术发明开始，到2005年第二代测序技术问世这段时间，DNA测序主要采用Sanger测序技术。

The rapid development of next-generation sequencing technologies makes the large scale genomes study possible . With the process of the Thousand Genomes Project , more and more individual human genomics will be sequenced .

随着新一代测序技术的发展和千人基因组计划的进行，越来越多的个人基因组序列已经被检测出来，个人基因组的数据也在飞速增长，数据量非常庞大。

Therefore , in order to understand the gene function and the role genes played in physiological and metabolic activities , doing research on gene expression of cotton with next-generation sequencing has become a hotspot in recent years .

因此，为了解读基因的功能，更好的研究基因在棉花生理代谢活动中的作用，利用新一代测序技术对棉花基因表达进行研究已成为近些年来的研究热点。

Transcriptional regulation is mediated by transcription factor via interaction with specific elements . Nevertheless , the application of next-generation sequencing ( NGS ) technologies can be used to determine gene sequences and transcript abundance and permit large-scale analyses of gene expression patterns despite the lack of a reference genome .

转录调控是通过转录因子和特殊元件相互作用来进行调解的，而新一代测序技术的应用能够确定基因序列和转录本，甚至在没有参考基因的情况下允许基因表达模式的大量分析。

But advances such as gene editing and next-generation DNA sequencing allow scientists to weaponise new viruses , perhaps including custom pathogens engineered to overcome existing immunities or to be impervious to current drugs .

但基因编辑和新一代DNA测序等技术进步使得科学家可以把新病毒变成武器，或许包括用定制病原体攻破现有免疫系统或抵抗现有药物的影响。

As the rapid development of the next-generation genome sequencing tech-nology , most bioinformatics studies has been also developed rapidly , including the TF regulation , microRNA , epigenetics , and de nono assembling as well as meta-genomics .

随着第二代测序技术的发展，大多数生物信息学领域也随之飞速发展，例如TF调控，microRNA，表观遗传学，全基因组组装，以及元基因组学等等。